Specialist for Sophisticated Antibody Development
Specialist for Sophisticated Antibody Development

Immunization Protocols of Rabbits using Peptides

Protocol Antigen synthesized by BioGenes Products sent to the customer Expected time frame
High Speed Peptide Peptide (15 mg, purity >80%), assistance in sequence selection and conjugation to a carrier 1.5 mL pre-immune serum and 20 mL antiserum per rabbit, one aliquot of the peptide 10 – 11 weeks after ordering
High Quality Peptide Peptide (15 mg, purity >80%), assistance in sequence selection and conjugation to a carrier 1.5 mL pre-immune serum and 40 mL antiserum per rabbit, one aliquot of the peptide 12 – 13 weeks after ordering
Complete & Easy Peptide Peptide (15 mg, purity >80%), assistance in sequence selection and conjugation to a carrier 1.5 mL pre-immune serum and approx. 90 mL antiserum per rabbit, remaining peptide 17 weeks after ordering
Peptide Mix Two peptides (each 15 mg, purity >80%), assistance in sequence selection and conjugation to a carrier 1.5 mL pre-immune serum and 20 mL antiserum per rabbit, one aliquot of each peptide 10 – 11 weeks after ordering
Phospho-Peptide Phosphorylated and corresponding non-phosphorylated peptide (15 and 10 mg, respectively, purity >80%), conjugation to a carrier 1.5 mL pre-immune serum and affinity purified anti-phospho-peptide and anti-non-phospho-peptide antibodies, one aliquot per peptide 14 weeks after ordering

High Speed Peptide

  • Peptide synthesis (15 aa, 15 mg, at least 80% purity) 
  • Conjugation of peptide to a protein carrier
  • Immunization of two rabbits according to a schedule over 42 days
  • Delivery of pre-immune serum and 20 mL antiserum per rabbit
  • Quality control of the antisera using ELISA 
  • The animals will be kept after delivery of the antiserum

High Quality Peptide

  • Peptide synthesis (15 aa, 15 mg, at least 80% purity) 
  • Conjugation of peptide to a protein carrier
  • Test of pre-immune sera and selection of suitable animals 
  • Immunization of two rabbits according to a schedule over 56 days 
  • Quality control of the antisera using ELISA 
  • Delivery of pre-immune serum and 40 mL antiserum per rabbit 
  • The animals will be kept after delivery of the antiserum

Complete & Easy Peptide

  • Peptide synthesis (15 aa, 15 mg, at least 80% purity, quality control by HPLC, MS)
  • Conjugation of peptide to a protein carrier
  • Immunization of two rabbits according to a schedule over 91 days 
  • Quality control of the antisera (first bleeding) using ELISA 
  • Delivery of pre-immune serum, 80-100 mL antiserum per rabbit 

Please note: This protocol cannot be altered, and the rabbits are not further kept!

Peptide Mix

  • Peptide synthesis of two peptides (15 aa, 15 mg, purity at least 80% each) 
  • Conjugation of peptides to a protein carrier
  • Immunization of two rabbits according to a schedule over 42 days
  • Delivery of pre-immune serum and 20 mL antiserum per rabbit
  • Quality control of the antisera using ELISA (both peptides)
  • The animals will be kept after delivery of the antiserum

Phospho-peptide

  • Synthesis of the phosphorylated peptide (12 aa + one phosphorylation, 15 – 20 mg, at least 80% purity, quality control by HPLC and MS)
  • Synthesis of the corresponding non-phosphorylated peptide (12 aa, 10 mg, at least 80% purity, quality control by HPLC and MS)
  • Conjugation of the phosphorylated peptide to a protein carrier 
  • Immunization of two rabbits according to a schedule over 42 days
  • Taking of pre-immune serum and 20 mL antiserum per rabbit
  • Quality control of the antisera using ELISA against the phosphorylated peptide
  • Affinity purification of up to 2 x 20 mL antiserum using three affinity columns: one loaded with Protein A, one loaded with the phosphorylated peptide and loaded with the unphosphorylated peptide
  • Delivery: samples of peptides, pre-immune serum, test serum, specific anti-phophopeptide and anti-non-phosphopeptide antibodies
  • The animals will be kept after delivery of the antibodies

Please note: usage of shorter or longer peptides is possible upon specific request!